Metagenomic insights into the alteration of soil N‐cycling‐related microbiome and functions under long‐term conversion of cropland to <i>Miscanthus</i>

Miscanthus spp. show excellent application prospects due to its bioenergy potential and multiple ecological services. Annual N export with biomass harvest from Miscanthus, even without fertilizer supplement, do not reduce soil levels. The question arises regarding how can maintain stable Metagenomic strategies were used reveal N-cycling-related microbiome their functional contributions processes of N-cycling based on the comparison among bare land, cropland, 10-year × giganteus, 15-year sacchariflorus fields. results showed that, after long-term cropland-to-Miscanthus conversion (LCMC), 16 21 bacterial phyla all archaeal exhibited significant changes. Soil microbial denitrification nitrification functions significantly weakened, fixation (NF) was enhanced. biosynthesis amino acids, especially alanine, aspartate, glutamate metabolism, in dramatically promoted. genus Anaeromyxobacter contributed largely NF process LCMC. Variations available potassium, N, organic C, total contents drove a shift cropland pattern. We conclude that recruit communities enhance benefiting sustainability balance.